Bacteriological profile

Diagnosis of bacterial agents of animal disease involves a range of techniques to take into account, as far as possible, the different types of biological material and the species of animal(s) from which the sample is derived.

Highly specific methods for the identification of pathogens based on a combination of mass spectrometric analysis (MALDI-TOF) and molecular genetic diagnostics (PCR), including sequencing of parts of the microbial genome, are also an integral part of these procedures.


MVDr. Tomáš Černý
+420 770 118 912


In the following text we would like to introduce you to the most common types of biological material examined and the methods of their collection:

  • Dead/lost animal - necropsy (with sampling) is performed at the Department of Pathology, SVU Prague.
  • Dead/lost animal, necropsy performed in the field, sanitation company - send for examination properly packaged tissue samples of 5-10 cm3 from the interface of healthy and diseased parts.
  • Organ swabs from field necropsy - send swabs in activated charcoal transport medium (AMIES) for examination.
  • Swabs from body orifices (ear, nose, throat, eye, rectum, cloaca, urogenital tract) - send swabs in activated charcoal transport medium (AMIES) for examination.
  • Skin scraping
  • Foot, hoof and claw swabs - send swabs in activated charcoal transport medium (AMIES) for examination. It is essential that anaerobic conditions are maintained at all times.
  • Intestinal contents (faeces, ruminal fluid) - send samples for examination in sterile tubes or wide-mouth samplers. It is essential that anaerobic conditions are maintained (e.g. by filling the tube to the brim).
  • Body fluids (urine, milk, sputum, semen) - send samples for testing in sterile tubes or wide-mouth specimen collection tubes.
  • Aspirated body fluids (cerebrospinal fluid, synovial fluid, blood, lymph, bone marrow, pathological abdominal and thoracic contents, genital lavage, respiratory lavage, pus, etc.) - send the collection set used to collect the fluid (blinded syringe, vacuum system, cannula, etc.) for examination. Always maintain anaerobic conditions.


MVDr. Eva Patrasová
+420 770 147 408


MVDr. Kateřina Kučerová
+420 770 118 907


MVDr. Ilona Parmová
+420 770 118 908


Typ souboru Název Velikost
General application form 0,1 MB Stáhnout
General application form 0,4 MB Stáhnout


How long can I store the sample before sending it to the laboratory?

Most manufacturers of AMIES activated carbon transport media guarantee that the microbiological composition of the sample remains unchanged for 48 hours. A temperature range (3 - 25 °C) should be maintained during storage and transport of samples.

To what extent and from which samples do you perform direct molecular genetic diagnostics of bacterial pathogens? Is it performed as part of routine bacteriological testing?

We currently offer a wide range of direct PCR methods for the detection of the causative organism. The most commonly requested tests are

- Mycoplasma detection 
- Chlamydia and Chlamydophila detection
- Detection of Taylorella equigenitalis 
- Detection of dermatophytes 
- Detection of foaling pathogens
- Detection of Lawsonia spp. and Brachyspira spp.

For these tests, it is necessary to send directly the affected tissue (organ, fluid, digestive tract contents, etc.) or a smear on an activated charcoal-free swab. The above-mentioned tests are only performed by PCR on the basis of a direct order from the client. Please do not hesitate to contact us if you have any questions regarding the diagnosis of other pathogens.

Do you perform pathogenicity factor testing on Escherichia coli and Clostridium perfringens isolates found in livestock?

Yes, we routinely test for the presence of adhesion factors in Escherichia coli by rapid slide agglutination. Further molecular genetic detection of genes encoding pathogenic factors is carried out in consultation with the customer. For Clostridium perfringens isolates, we are able to perform molecular genetic detection of the toxin production genes alpha, ß1, ß2, ε, γ at the customer's request.

Do you perform antimicrobial susceptibility testing on pathogens?

Yes, we do. For bacterial pathogens, we can perform antimicrobial susceptibility testing using the disc diffusion method or the microtitre dilution method to determine the minimum inhibitory concentration (MIC).