Paratuberculosis (Johne's disease) is a chronic inflammation of the small intestine of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP).


The clinical signs of paratuberculosis in cattle are slowly progressive weight loss and diarrhoea, which is initially intermittent and gradually becomes more severe until it is permanent. This is similar in farmed deer. Diarrhoea is less common in small ruminants.

We offer serology, culture, bacterioscopy and PCR testing.

Bacterioscopic examination for acid-alcohol resistant rods by Ziehl-Neelsen staining is a rapid indicative method suitable for faecal and autopsy samples. Bacterioscopy is always performed with culture, but can be ordered separately.

The culture examination is carried out on faeces or autopsy material. It is the most sensitive method. The duration of the test is 3 months. In case of a positive result, the grown colonies are confirmed by PCR.

PCR confirmation is performed on faeces or section material. The method is suitable for confirmation of the disease at clinical manifestations of the disease.

Antibody testing is available in our laboratories.


MVDr. Ilona Parmová
+420 770 118 908


MVDr. Eva Patrasová
+420 770 147 408

Virology and serology

MVDr. Roman Masopust
+420 770 118 901

Molecular biology

RNDr. Alexander Nagy, Ph.D.
+420 770 118 897


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What material is suitable for testing?

- Blood for serological examination or fresh faeces are sent for examination of live animals.

- At necropsy or at post-mortem examination, a portion of the small intestine (ileum, ileocecal valve) and an intestinal lymph node are taken.