Diagnosis of bacterial agents of animal disease involves a range of techniques to take into account, as far as possible, the different types of biological material and the species of animal(s) from which the sample is derived.
Highly specific methods for the identification of pathogens based on a combination of mass spectrometric analysis (MALDI-TOF) and molecular genetic diagnostics (PCR), including sequencing of parts of the microbial genome, are also an integral part of these procedures.
In the following text we would like to introduce you to the most common types of biological material examined and the methods of their collection:
| Typ souboru | Název | Velikost | |
|---|---|---|---|
| General application form | 0,1 MB | Stáhnout | |
| General application form | 0,4 MB | Stáhnout |
Most manufacturers of AMIES activated carbon transport media guarantee that the microbiological composition of the sample remains unchanged for 48 hours. A temperature range (3 - 25 °C) should be maintained during storage and transport of samples.
We currently offer a wide range of direct PCR methods for the detection of the causative organism. The most commonly requested tests are
- Mycoplasma detection
- Chlamydia and Chlamydophila detection
- Detection of Taylorella equigenitalis
- Detection of dermatophytes
- Detection of foaling pathogens
- Detection of Lawsonia spp. and Brachyspira spp.
For these tests, it is necessary to send directly the affected tissue (organ, fluid, digestive tract contents, etc.) or a smear on an activated charcoal-free swab. The above-mentioned tests are only performed by PCR on the basis of a direct order from the client. Please do not hesitate to contact us if you have any questions regarding the diagnosis of other pathogens.
Yes, we routinely test for the presence of adhesion factors in Escherichia coli by rapid slide agglutination. Further molecular genetic detection of genes encoding pathogenic factors is carried out in consultation with the customer. For Clostridium perfringens isolates, we are able to perform molecular genetic detection of the toxin production genes alpha, ß1, ß2, ε, γ at the customer's request.
Yes, we do. For bacterial pathogens, we can perform antimicrobial susceptibility testing using the disc diffusion method or the microtitre dilution method to determine the minimum inhibitory concentration (MIC).
