Skin scrapings - bacteriological diagnosis

A proper examination of skin scrapings requires a comprehensive approach including basic direct microscopic mycological and parasitological diagnosis, bacteriological and mycological culture. This is followed by typing of the pathogens by MALDI-TOF, complemented by direct detection of dermatophytes by PCR. Bacterial pathogens are also tested for their susceptibility to antimicrobial agents.


Microscopic diagnosis allows the presence of spores of dermatophytic fungi infecting the hair to be detected and also reveals the possible presence of ectoparasites in the skin scrapings.

Bacteriological and mycological cultures allow the detection of the entire spectrum of bacterial and mycotic agents (including yeasts) causing skin diseases and skin derivatives (claws, cornices, etc.).

Due to the difficulty and time consuming nature of conventional mycological culture of dermatophytic fungi, we also offer molecular genetic detection of these fungi directly from skin scrapings. In case of a positive result, we can also perform genus and species identification by genome sequencing analysis (MicroSeq).


MVDr. Tomáš Černý
+420 770 118 912


MVDr. Kateřina Kučerová
+420 770 118 907


MVDr. Eva Patrasová
+420 770 147 408

Molecular biology

RNDr. Alexander Nagy, Ph.D.
+420 770 118 897

Molecular biology

Ing. Lenka Černíková, Ph.D
+420 770 118 896


Ing. Ondřej Máca, Ph.D.
+420 770 119 013


Typ souboru Název Velikost
General application form 0,1 MB Stáhnout
General application form 0,4 MB Stáhnout


How do I properly remove the skin scrapings and what medium should I use to collect and transport the samples?

For testing, samples of hair, crusts or pathological changes should be taken from the interface between the healthy and affected areas. The actual scraping is best done with a scalpel blade until the first capillary bleeding appears. Place the material obtained in a sterile tube or wide-mouth specimen cup and send for examination.

Is it possible to order only mycological or parasitological testing of a swab?

Yes, you can specify in your order if you want a complete examination or only some parts of it (bacteriological, mycological, parasitological or PCR examination) or any combination of them. If you require a full test, you must send a sufficient amount of sample (1-2 g) to allow these tests to be carried out.

How long does it take to culture a skin scraping for dermatophytes?

The usual duration of the culture examination is between 14-21 days. In the case of slow-growing dermatophytes detected in large ruminants (Trichophyton verrucosum), the culture time may be extended to 5-7 weeks. Molecular genetic detection of these fungi directly from skin scrapings is performed within 3-7 days.